The medium was then removed from the wells, and 100 µl of DMSO was added to solubilize the formazan crystals. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Published Dec 16, 2020 | Updated Dec 16, 2020. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. Table of Contents. Description 1. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. Standard features include a cuvette port,. SpectraMax® M4). 12. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Table 2. For labeling representative cells, they were treated under the same conditions as above, followed by fixation with 2% paraformaldehyde for 20 min at room temperature. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. . overnight. Background-subtracted data values (mean +/- SD) are graphed. 5. e. Measurement of Mitochondrial Membrane Potential by JC-1. 2. Introduction. 1 mg·mL −1 (Sigma-Aldrich, USA) as described above and fluorescence intensity at λ ex = 530 nm and λ em = 590 nm was detected through a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA). Microscopic evaluation of apoptosis. Three kits covering a wide range of dsDNA concentrations. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. SpectraMax Gemini XPS/EM Register or Sign In. Compare plate readers7 Chapter 1: Installation and Setup SoftMax Pro Microplate Data Acquisition and Analysis Software controls Molecular Devices spectrophotometers, absorbance, luminescence, and fluorescence microplate readers, and the SpectraMax Paradigm Multi-Mode Detection Platform. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. TSQ was dissolved in Lock’s buffer (pH 7. 05 mg mL −1. , UK) or SpectraMax M4 by Molecular Device. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. 2. 5 mg mL −1 with 0. 3752 in as per SBS dimensions. Environmental Chamber and Respiratory SimulatorsRhizoma drynariae, a traditional Chinese herb, is commonly used in treatment of bone healing in osteoporotic fractures. 05 mg mL −1. AM (~0. SPECTROMAXx with iCAL 2. Note: In this user guide, SpectraMax ® refers to several SpectraMax . The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. spr SpectraMax iD5 SpectraTest ABS2 v1. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Kinetic fluorescence measurements were taken for 45 min at 37 °C in a SpectraMax M4 microplate reader (540 nm ex /590 nm em). For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. SpectraMax M Series Multi-Mode Microplate Readers | MolecularThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Where can I find UV/Vis Plastic Disposable Cuvettes? Some Plastic Disposable UV (300 nm +) and VIS Cuvette sources: NOTE: Some disposables with channel sides (as opposed to full. In the M2, M5 and Plus. 4 kilograms). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 This document is provided to customers who have purchased Molecular Devices,…spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485nm and emissionλ 535nm. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Transfected cells were seeded into 6-well plates at a density of 200 cells/well. FlexStation™ III. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). SpectraMax® Aquamax. 03 in x 3. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. (Shanghai, China). Serum C-terminal telopeptide of type I collagen (S-CTX), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRAP) levels in serum and the receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), and RANK levels in marrow were determinedThe ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Description 1. Inhibition of CPT-11 Hydrolysis by Four Bioflavonesfor the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. item 7 replacement bulb for molecular devices spectramax m4 flash lamp replacement bulb for molecular devices spectramax m4 flash lamp. DNA standards ranging from 50 pg/mL to 1 μg/mL were detected using the Quant-iT PicoGreen dsDNA Assay Kit and SpectraMax readers (data from the SpectraMax iD5 reader are shown, but other SpectraMax readers gave similar results). SpectraMax M2/M2e reader: TRUE: Molecular Devices: Aquamax DW4 washer: TRUE: Molecular Devices: SpectraMax M4 reader: TRUE: Molecular Devices: SpectraMax M5/M5e reader: TRUE: Molecular Devices:. D 1 1. 116 SpectraMax M5 and M5e Multi-Mode. Allows you to use EOS lenses on a micro four thirds camera M4/3 K&F concept lens adapter. The optical systems Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . The optical density at 450 nm was detected using a microplate reader (SpectraMax M4, MolecularDevices, CA, USA). alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. , San Jose, CA) was. . org) with optimized instrument settings (Table 1) were used to generate and analyze data. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. Click Read. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. For SpectraMax L or LMax II, LMax II 384 - no adapter used. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Roelli1, Amandine Stoss1, Rebecca A. Dettmer4, Wayne A. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Like all. 0 calibration logic, which needs only 5 minutes and a single sample per day rather than the 30 or more minutes needed by conventional analyzers. Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). 37. Microplate Readers include the M3, M4,. 3. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). 72 MB. Figure 1. ). In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. Choose from a three- (M3), four- (M4), orCables. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. However, dynamic changes in soil microbiota under different tree ages have received little attention. 95. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. Parameter SpectraMax iD5 SpectraMax i3x. 9000-0756. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Pentobarbital sodium and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. High-performance liquid chromatography (HPLC) chromatograms were performed on Agilent Infinity 1260 with a C18 column (100 Å, 30 × 4. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. After incubation, the absorbance value of all samples was measured at 517 nm on a Spectramax M4. Quick, Simple Standardization: The new analyzer features SPECTRO’s proprietary iCAL 2. Microplate Reader Shelf. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Parameter SpectraMax iD5 SpectraMax i3x SpectraMax. The IC50 curves were calculated using variable slope for log (inhibitor) vs. Three kits covering a wide range of dsDNA concentrations. The drawing shows a standard microplate of 15 mm height covered with a lid. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. To avoid injury, it is recommended that two. Expand the ABS1 Plate Inserted or Expt#3 experiment and select the OptAlign&UltDrk Plate section. For more detailed information and technical support of Molecular Devices instruments or software,Luciferase Detection Instrumentation Guide. 303 unable to cal dark current. WARNING! The instrument weighs approximately 36 pounds (16. We would like to show you a description here but the site won’t allow us. Each well was scanned at nine evenly distributed points and averaged automatically with the software. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. However, little is known about the regulatory mechanisms of the degradative pathway. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf, Switzerland). Other technologies such as imaging. . book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Interpolated concentrations of native CRP in human serum and plasma samples. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. VIEW DETAILS The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. Record fluorescence emission data in kinetic mode for 2 min with 20 s between reads to ensure that interaction has reached equilibrium and take average. Choose from a three- (M3), four- (M4), or We would like to show you a description here but the site won’t allow us. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. In this study, changes in soil physicochemical properties, as well as bacterial diversity and community structures (by high-throughput. Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. The plate reader. 9000-0756. Home; Lighting; Work lights; User manual. SpectraMax® M5. 7. Dissolve FITC-dextran in PBS at a. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). , and red fluorescence at 543 ex. 2. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. FlexStation™ II. 6: bioNova científica, s. 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). ® reader to the computer. F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4Relative florescence units (RFU) were quantified on a plate reader (SpectraMax®; M4; Molecular Devices, Sunnyvale, CA) every 1 minute over a 10‐minute period at 37°C, using excitation and emission wavelengths of 328 and 420 nM, respectively. For SpectraMax L or LMax II, LMax II. 7 cells. When the user applies a set temperature of 37°C, the top is heated to 37. Bottom-reading is usually recommended for cell-based assay with adherent cells. * Nunc. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. 7°C and the bottom to 36. Finally, we have two New Brunswick Excella E24 shaking incubators, one with refrigeration capabilities and a Thermo Sciences -80˚C freezer. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. 0397 in x 3. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. ZERO BIAS - scores, article reviews, protocol conditions and moreNote: For the SpectraMax M2, M2e, M3, M4, M5, M5e, and FlexStation 3. Note: In this user guide, all references to SpectraMax Multi-Mode. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Other technologies such as imaging. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. MTT values at day 3 were normalized to day 0. After 48 h, the optical density (OD) was measured at 450 nm using the microplate reader (SpectraMax M4). Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Establishment of mouse orthotopic xenograft bladder tumor model. 1. After aerosol collection was completed, the impactor plates were rinsed with 0. All experiments. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. At the end of the incubation period, MTT was added and the plates were incubated for 4 h at 37°C. After preparation, wrap in foil (see Note 1). Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. MTT assay was utilized to detect the cytotoxicity of different compounds on HUVECs. The SpectraMax M2 and M2e are versatile and reliable. S. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 9 pg/mL. book Page 1 Wednesday, July 7, 2010 2:09 PMTo quantify the cell density of bacterial cells, a SpectraMax M4 Microplate Reader was used to take measurements of the optical density at 600 nm. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Plate Formats: 6, 12, 24, 48, 96, 384 Well Plates. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. 2. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. 05 mol/L hydrochloric acid and 0. SpectraMax® M5e. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. We have 2 Molecular Devices SpectraMax M4 manuals available for free PDF download: User Manual . The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Optical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). to View Figures. The combination index (CI) was calculated. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax Spectrofluorometers. The ability of the liposome to be internalized into cell was assessed using cell culture studies 45-47. /600 em. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. 2 only). 次いで、ユーザーのラボに. Call 516-939-0888 to check current. Description 1. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). Table 2. 313 reference gain check fail. 7°C (gradient heating design). Cellular viability was assessed by using resazurin at 1. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Add To My List; Create New List; Quickview. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] number of moles of FITC conjugated to OVA was determined using UV–vis spectroscopy (SpectraMax M4, Molecular Devices Inc. Bioz Stars score: 98/100, based on 1 PubMed citations. 2 pM 2 pM 384-well (50 µL) 3 pM 4 pMCell viability was determined by addition of MTT ([3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma) to cells and incubation for 4 h. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. . SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. TSQ was dissolved in Lock’s buffer (pH 7. (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities for Detection Estimates, Magnitude 1 through 6. All configurations. 90-0083 series): Excitation 530 nm,. MIC assay: After diluting colloidal nanoparticle solutions of varying concentrations in TSB, they were injected onto a 96-well plate with a strain of bacteria at a concentration of 10 6 CFU/mL. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. SpectraMax® M4). † Bottom mounting for SpectraMax Paradigm Readers only. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. Control AM were incubated in the same buffer for 1 h at 37 °C. SpectraMax i3(x) SpectaTest ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. 1step assay with SpectraMax microplate readers. Examples: “Press carefully to engage,” “ Do not press down. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Choose cables that meet the requirement described in this appendix. > spectramax m4 microplate reader. In. 1% CF3COOH) = 5/95 (v/v) to 95/5 (v/v); flow rate:The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. Background-subtracted data values (mean +/- SD) are graphed. Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). The following document is intended to demonstrate setup of this instrument. Pierce™ BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf,. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Results were expressed as a percentage relative to the control group (defined as 100%). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. , cyan fluorescence at 458 ex. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples. 2. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. . Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. 2. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 01) + 0. Description 1. 316 grating motor driver faulty. After 7 days of culture, the colonies generated. The activity of untreated cells was normalized to 100% and the treated cell activity was compared with that. Detection modalities are shown in Table 1-1. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Description 1. All configurations. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. 4. To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. RFU was plotted versus time to derive the rate of formation of the fluorophore. 5. Page: 1/6. Test samples were measured according to the manufacturer’s instructions using a spectrophotometer SpectraMax M4 (Molecular Devices, Sunnyvale, CA). The following document is intended to demonstrate setup of this instrument. 2. SigmaPlot (Systat Software, Inc. 305 reference level saturation. e. The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). StakMax Handling Stacker Base Unit. Statistical. Multi-Mode Microplate Readers. The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). 7. Note: In this user guide, all references to SpectraMax Multi-Mode. . Data are from ≥5 independent experiments. The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). M2 M2e M3 M4 M5 M5e ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Detection modalities are shown in Table 1-1. After incubation, the fluorescence intensity was measured using Spectramax M4 (Molecular Devices) (excitation: 380 nm emission: 460 nm). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Choose from a three- (M3), four- (M4), or Qualify the luminescence performance of the SpectraMax . While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The lower critical solution temperature (LCST) at which phase transition occurs was measured via plate reader absorbance for n = 3 samples (SpectraMax M4; Molecular Devices). VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Plot the fluorescence intensity versus the cell seeding number. +1 250-415-0785. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The test conditions are as follows: mobile phase: acetonitrile/H2O (0. Highly selective for dsDNA over ssDNA or RNA. 4) and cells were stained with 150 nM TSQ for 1 min. Detection modalities are shown in Table 1-1. Highly selective for dsDNA over ssDNA or RNA. 2. Molecular Devices SpectraMax® series microplate readers. Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range.